2nd INTEGRATIVE STRUCTURAL BIOLOGY MEETING

November 29th to December 3rd 2021

BSI2021

Program and Schedule

program overview

Detailed Scientific program

Monday 29th November

13:00 Welcome

13:45 DNA world

Chair: Dr TIMMINS

Valérie LAMOUR, IGBMC, Illkirch (30 min)
Romain KOSZUL, Institut Pasteur, Paris (30 min)
Marcin J. SUSKIEWICZ, CBM, Orléans (20 min)

Structural and mechanistic insights into PARP1 and PARP2 function and PARP inhibitor response

Xavier MANIVAL, IMOPA, Nancy (20 min)

The box C/D snoRNP assembly factor Bcd1 interacts with the histone chaperone Rtt106 and controls its transcription dependent activity

Claire CARGEMEL, I2BC, Gif-sur-Yvette (20 min)

DciA, the ancestral replicative helicase loader

15:45 Keynote

Andrea CARFI, Moderna

16:15 Coffee break

15:30 RNA world

Chair: Drs Nollmann and Gervais

Thomas GREGOR, Institut Pasteur, Paris (30 min)
Emmanuelle SCHMITT, Ecole polytechnique, Palaiseau (30 min)

Translation initiation in Archaea

Can WANG, BIOC, Ecole polytechnique, Palaiseau (20 min)

Structural and functional insights into Archaeoglobus fulgidus m2G10 tRNA methyltransferase Trm11 and its Trm112 activator

Carine TISNE, IBPC, Paris (20 min)

Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs

Julien MARQUEVIELLE, IECB/ARNA, Bordeaux (20 min)

NMR study of a RNA G-quadruplex forming sequence within HIV-1 U3 region

Evening : Welcome cocktail

Tuesday 30th November

8:30 Macromolecular Assemblies

Chair : Drs PLISSON-CHASTAING et COUREUX

Stéphane ROCHE, I2BC, Gif-sur-Yvette (20 min)

High Resolution CryoEM Structure and Assembly Mechanism of a Bacterial Virus Genome Gatekeeper

Lu YANG, Institut Pasteur, Paris (20 min)

The actinobacterial exception: 2.3 Å resolution cryo-EM structure of a large enzymatic machinery from a central metabolic supercomplex

Sayma ZAHID, I2BC, Gif-sur-Yvette (20 min)

Structural and functional study of the complex formed by the Werner protein (WRN) and the Ku70/Ku80 heterodimer

Helgo SCHMIDT, IGBMC, Illkirch (20 min)

The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1

10:30 Coffee break

11:00 Health issues I

Chair: Dr Gaudin

Juan REGUERA, AFMB, Marseille (30 min)
Rémi FRONZES, IECB, Bordeaux (30 min)
Rania GHOUIL, I2BC, Gif-sur-Yvette (20 min)

A cryptic motif in BRCA2 triggers oligomerization of the meiotic HSF2BP, as characterized by NMR, X-ray crystallography and cryo-electron microscopy

Xavier HANOULLE, Integrative Structural Biology Lab, Lille (20 min)

NMR of SARS-CoV-2 Main Protease (3CLpro) for drug development

Célia CAILLET-SAGUY, Institut Pasteur, Paris (20 min)

Host PDZ-containing proteins targeted by Hepatitis B virus and SARS-CoV-2

13:00 Lunch

13:45 Poster session

14:30 Sponsor presentation

15:00 Keynote

Jonas RIES, EMBL, Heidelberg, Germany

Superresolution microscopy for structural cell biology

15:30 Formation en continue

Chair: Dr Le Du

Sandrine DELACROIX-MORVAN, France Université Numérique
Fabrice CORDELIERE, France Bio Imaging
Dominique HOUSSET, French Infrastructure for Integrated Biology

16:30 Discussion

Animée par Cécile Michaut

Wednesday 1st December

8:30 Integral & peripheral membrane proteins

Chair: Drs Margeat and Czjzek

Agata NAWROTEK, ENS Paris-Saclay, Gif-sur-Yvette (30 min)

Regulation of small GTPase signaling at the periphery of membrane

Vincent CHAPTAL, IBCP, Lyon (20 min)

CryoEM reconstructions of membrane proteins solved in several amphipathic solvents, Nanodisc, amphipol and detergents, yield amphipathic belts of similar sizes corresponding to a common ordered solvent layer

Laurent CATOIRE, IBPC, Paris (20 min)

Study of the dynamic interplay between lipids and membrane proteins by hydrostatic pressure

Robert QUAST, CBS, Montpellier (20 min)

Dissecting the allosteric modulation of the Metabotropic Glutamate Receptor 2 using single molecule FRET

Guillaume Lebon, IGF, Montpellier (20 min)

Structural basis of the human mGlu5 receptor activation mechanism

10:30 Coffee break

11:00 Non-membrane compartmentalization

Chair: Dr Ruff

Andrea PARMEGGIANI, L2C, Montpellier (30 min)
Stefan AROLD, KAUST, Thuwal, Arabie Saoudite (20 min)

Kinase control through a highly disordered dimerization and calmodulin-binding element

Quentin NEVERS, I2BC, Gif-sur-Yvette (20 min)

Identification of structural elements required for the formation of rabies virus phosphoprotein and nucleoprotein inclusions in different minimal systems

Christina SIZUN, ICSN, Gif-sur-Yvette (20 min)

A structural and dynamic analysis of a viral platform protein

Marjolaine NOIRCLERC-SAVOYE, IBS, Grenoble (20 min)

HIV-1 Rev bound to human Importin beta : an integrative study of a protein complex

13:00 Lunch

13:45 Poster session

14:30 Sponsor presentation

15:00 Keynote

Simon ALBERTI, Max Planck Institute, Dresden, Germany

Biomolecular condensates at the nexus of cellular stress, aging and disease

15:30 Drug design

Chair: Dr Berger

Maria DUCA, ICN, Nice (30 min)
Axel INNIS, IECB, Bordeaux (30 min)

Context-dependent inhibition of protein synthesis by ribosome-targeting antibiotics

Laurent MAVEYRAUD, IPBS, Toulouse (20 min)

Inhibition of mycobacterial phosphopantetheinyl transferases

Magali MATHIEU, SANOFI, Vitry-sur-Seine (20 min)

KRAS G12C fragment screening renders new binding pockets

Eric ENNIFAR, Unistra, Strasbourg (20 min)

New drug design opportunities provided by Thermodynamics of Macrolide and PrAMP antibiotics binding to the bacterial ribosome provide

17:30 Discussion

Olivier NOSJEAN, Research Scientific Director, Servier, Croissy-sur-Seine

Darren HART, FRISBI, director Grenoble Center and Director of Integrated Structural Biology Grenoble (ISBG), IBS, Grenoble

Hélène CHAUTARD, Directrice du pôle science de la vie, de la Direction de l’Investissement de la SATT Paris-Saclay

Claude Nogues, Directrice Scientifique de Kimialys, Paris

Thursday 2nd December

8:30 Health issues II

Chair: Drs Pedelacq and Izadi

Alain MARTY, LISBP, Toulouse (30 min)
Oriane FRANCES, Sanofi, Vitry-sur-Seine (30 min)
Marc LENSINK, Lille (20 min)

The CAPRI covid-19 Open Science Initiative

Faustine HENOT, IBS, Grenoble (20 min)

Human HSP90 a contortionist drug target : Modulation of its energy landscape upon ligand binding

Carlos A. ELENA-REAL, CBS, Montpellier

High-resolution characterization of pathogenic and non-pathogenic Huntingtin protein poly-Q tracts

10:30 Coffee break

11:00 Methodologies I

Chair: Drs Gabelica and Czjzek

Sabine HEDIGER, IRIG, Grenoble (30 min)

Unraveling binding sites by selective DNP-enhanced NMR

Claude SAUTER, Unistra, Strasbourg (20 min)

Serial crystallography: a game-changer in crystallization and crystal handling

Martin WEIK, IBS, Grenoble

Photoenzyme at work: Structural dynamics of fatty acid photodecarboxylase

Marie LEY, Unistra, Strasbourg

Integration of mass spectrometry data to structural biology for the characterization of PRMT2/RSF1 interaction

12:30 Keynote

Irina GUTSCHE, IBS, Grenoble

13:00 Lunch

13:45 Poster session

14:30 General assembly

15:00 SFB Prize 2019

Emmanuelle BIGNON, Biochemistry Department, Université Nice Sophia Antipolis, Nice

Understanding the dynamics and reactivity of nucleic acids to fight against pathologies

Sélection 2021
15:30 Science et société

Chair: Dr BONTEMS

Evening : Gala dinner

Friday 3rd December

8:30 Methodologies II

Chair: Dr Lescop

Florent DELHOMMEL, Bavarian Nmr Center, Technical University Of Munich / Institute Of Structural Biology, Germany (20 min)

NMR and structural characterization of NudC, a co-chaperone involved in client-transfer between heat shock proteins

Adeline ROBIN, IBS, Grenoble (20 min)

Fragment-based drug discovery on lactate dehydrogenase crystals grown in presence of Crystallophore.

Leo CHAVAS, Nagoya University, Synchrotron Radiation Research Center, Japan (20 min)

Combining expert methods for multi-scale and correlated analysis

Angela MANTOVANELL, IBS, Grenoble (20 min)

The Photoswitching Mechanism of the Fluorescent Protein rsEGFP2 at Cryogenic Temperature

10:00 Coffee break

10:30 Infrastructures

Chair: Dr Robert

Eric GUITTET,

Chargé de mission Secteur Biologie Santé, Ministère en charge de l’enseignement supérieur et de la recherche)

Daniel BOUJARD,

Directeur Adjoint Scientifique (DAS) du pôle Infrastructures nationales, Plateformes de l’INSB, CNRS

Jean-Pierre SIMORRE,

Délégué scientifique des grandes infrastructures de recherche de l’INC, CNRS

Bruno KLAHOLZ,

Responsable de l’infrastructure FRISBI

Carine VAN HEIJENOORT,

Responsable de l’Infrastructure InfrAnalytics

Béatrice SATIAT-JEUNEMAITRE,

Représentante FBI

13:00 Lunch

13:45 Conclusion

14:00-16:00 Satellite session Alphafold

Chair:

Randy READ
Laurent TERRADOT
Raphaël GUEROIS
Irène BARBARIN

Social program

In preparation